---
title: "Creates a primer"
method: POST
path: "/api/v1/primers"
tags: ["Primers"]
---

# Creates a primer

`POST /api/v1/primers`

Create a primer.

## Request body

- CreatePrimer
  - `token` string, required
  - `item` object
    - `title` string, required — The name of the primer
    - `alternative_name` string — Any alternative name by which the primer may be known
    - `owner_id` integer — id of the owner - if omitted, will default to your member id
    - `sequence` string — The nucleotide sequence of the primer
    - `tag` string — A unique label or tag associated with the primer
    - `gene_id` integer, nullable — A reference ID linking the primer to a specific gene in your lab
    - `tm` string — The melting temperature (Tm) of the primer
    - `orientation` string, nullable — Indicates the orientation of the primer. Set "1" for forward and "0" for reverse. This field is optional and can be left null if orientation is not applicable
    - `target_position` string — The specific binding position of the primer on the target DNA
    - `fragment_size` string — The size of the DNA fragment that the primer is designed to amplify
    - `organism` string — The specific organism for which the primer is designed, such as "Mouse"
    - `used_for` string — The specific applications for which the primer is used (pcr / RT / SDM / etc.)
    - `restriction_site` string — Information about any restriction enzyme recognition sites incorporated into the primer design
    - `source` string — The origin or source from which the primer was obtained
    - `description` string — A detailed description of the primer

## Response `201`

Created

## Other responses

- `401` — Error: Unauthorized
- `422` — invalid request

---

[API](https://skmtc.net/labguru/apis/labguru-api.md) · [All operations](https://skmtc.net/labguru/apis/labguru-api/llms.txt) · [OpenAPI document](https://skmtc-service-staging.skmtc.workers.dev/v1/apis/labguru/labguru-api/revisions/0534e4d70b77/schema)